Precise Mapping of the Brain α2-Adrenergic Receptor Gene within Chromosome 4p16
Identifieur interne : 004130 ( Main/Exploration ); précédent : 004129; suivant : 004131Precise Mapping of the Brain α2-Adrenergic Receptor Gene within Chromosome 4p16
Auteurs : Olaf Riess [Allemagne] ; Ulrike Thies [Allemagne] ; Ina Siedlaczck [Allemagne] ; Susanne Potisek [Allemagne] ; Rona Graham [Allemagne] ; Jane Theilmann [Allemagne] ; Tiemo Grimm [Allemagne] ; Joerg T. Epplen [Allemagne] ; Michael R. Hayden [Allemagne]Source :
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Abstract
The gene encoding the brain α2-adrenergic receptor (ADRA2C) is located on human chromosome 4. It has been circumstantially associated with a number of human disorders, including Parkinson disease, panic disorders, and Huntington disease (HD). Using somatic cell hybrids, we localized the gene to chromosome 4p16 distal to P8 (D4S62). To investigate this locus further, we isolated several cosmid clones covering the entire gene. The gene was found to be intronless. Two (GT)n repeats in close proximity to the ADRAC2 gene were analyzed and used to define its precise location. Linkage disequilibrium studies of one microsatellite in HD families showed strong nonrandom association to the HD mutation, indicating its tight linkage to the HD gene. The investigation of families carrying recombinant chromosomes, pulsed-field analysis, and genomic walking mapped the ADRA2C gene adjacent to D4S81, 500 kb proximal to the HD gene. The newly defined microsatellites at the ADRAC2 locus, its precise localization within 4p16, and the detailed PCR conditions facilitate the identification of any defect caused by this gene.
Url:
DOI: 10.1006/geno.1994.1061
Affiliations:
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Hayden</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Molecular Human Genetics, Ruhr University, Bochum, Federal Republic of Germany; Institute for Human Genetics, University of Goettingen, Federal Republic of Germany; Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada; and Institute for Human Genetics, University of Wuerzburg</wicri:regionArea><wicri:noRegion>University of Wuerzburg</wicri:noRegion><wicri:noRegion>University of Wuerzburg</wicri:noRegion></affiliation></author></analytic><series><idno type="ISSN">0888-7543</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0888-7543</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The gene encoding the brain α2-adrenergic receptor (ADRA2C) is located on human chromosome 4. It has been circumstantially associated with a number of human disorders, including Parkinson disease, panic disorders, and Huntington disease (HD). Using somatic cell hybrids, we localized the gene to chromosome 4p16 distal to P8 (D4S62). To investigate this locus further, we isolated several cosmid clones covering the entire gene. The gene was found to be intronless. Two (GT)n repeats in close proximity to the ADRAC2 gene were analyzed and used to define its precise location. Linkage disequilibrium studies of one microsatellite in HD families showed strong nonrandom association to the HD mutation, indicating its tight linkage to the HD gene. The investigation of families carrying recombinant chromosomes, pulsed-field analysis, and genomic walking mapped the ADRA2C gene adjacent to D4S81, 500 kb proximal to the HD gene. The newly defined microsatellites at the ADRAC2 locus, its precise localization within 4p16, and the detailed PCR conditions facilitate the identification of any defect caused by this gene.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country></list><tree><country name="Allemagne"><noRegion><name sortKey="Riess, Olaf" sort="Riess, Olaf" uniqKey="Riess O" first="Olaf" last="Riess">Olaf Riess</name></noRegion><name sortKey="Epplen, Joerg T" sort="Epplen, Joerg T" uniqKey="Epplen J" first="Joerg T." last="Epplen">Joerg T. Epplen</name><name sortKey="Graham, Rona" sort="Graham, Rona" uniqKey="Graham R" first="Rona" last="Graham">Rona Graham</name><name sortKey="Grimm, Tiemo" sort="Grimm, Tiemo" uniqKey="Grimm T" first="Tiemo" last="Grimm">Tiemo Grimm</name><name sortKey="Hayden, Michael R" sort="Hayden, Michael R" uniqKey="Hayden M" first="Michael R." last="Hayden">Michael R. Hayden</name><name sortKey="Potisek, Susanne" sort="Potisek, Susanne" uniqKey="Potisek S" first="Susanne" last="Potisek">Susanne Potisek</name><name sortKey="Siedlaczck, Ina" sort="Siedlaczck, Ina" uniqKey="Siedlaczck I" first="Ina" last="Siedlaczck">Ina Siedlaczck</name><name sortKey="Theilmann, Jane" sort="Theilmann, Jane" uniqKey="Theilmann J" first="Jane" last="Theilmann">Jane Theilmann</name><name sortKey="Thies, Ulrike" sort="Thies, Ulrike" uniqKey="Thies U" first="Ulrike" last="Thies">Ulrike Thies</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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